Anti-SOX2 Antibody (Clone#BIO-19)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4147

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-SOX2 antibody (BM4147). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOX2 antigen affinity purified monoclonal antibody (BM4147) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOX2 at approximately 36 kDa. The expected band size for SOX2 is at 34 kDa.
all(5)
IHC analysis of SOX2 using anti-SOX2 antibody (BM4147).
SOX2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-SOX2 Antibody (BM4147) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of SOX2 using anti-SOX2 antibody (BM4147).
SOX2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-SOX2 Antibody (BM4147) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of SOX2 using anti-SOX2 antibody (BM4147).
SOX2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SOX2 Antibody (BM4147) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
Immunofluorescent analysis of NCCIT cells, using SOX2 Antibody.
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-SOX2 Antibody (Clone#BIO-19)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

ANOP3; MCOPS3; SOX2; Transcription factor SOX 2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

SOX2

克隆号

BIO-19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human SOX2

计算分子量

34 kDa

实际分子量

34 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).

Uniprot ID

P48431

Gene ID

6657

文献引用格式

SOX2 Antibody (Clone#BIO-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4147)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

SRY-box transcription factor 2

蛋白名全称

Transcription factor SOX-2

实验方案>

实验步骤

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