Anti-Chk1/CHEK1 Antibody (Clone#AAF-3)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF IP

BM3968

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Chk1/CHEK1 antibody (BM3968). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse thymus tissue lysates,
Lane 7: mouse SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Chk1/CHEK1 antigen affinity purified monoclonal antibody (BM3968) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Chk1/CHEK1 at approximately 54 kDa. The expected band size for Chk1/CHEK1 is at 54 kDa.
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产品简介>

产品名称

Anti-Chk1/CHEK1 Antibody (Clone#AAF-3)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CHEK1; CHK1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, IP

基因名称

CHEK1

克隆号

AAF-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Chk1

计算分子量

54 kDa

实际分子量

54 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization, the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1 is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Furthermore, CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.

Uniprot ID

O14757

文献引用格式

Chk1/CHEK1 Antibody (Clone#AAF-3) (Boster Biological Technology, Wuhan, China. Catalog#BM3968)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Serine/threonine-protein kinase Chk1

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实验步骤

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