| Western blot (WB): | 1:500-2000 |

Western blot analysis of anti-LAMP1 antibody (BA2787-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAMP1 antigen affinity purified polyclonal antibody (BA2787-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LAMP1 at approximately 90-120 kDa (Glycosylation). The expected band size for LAMP1 is at 42 kDa.

Western blot analysis of anti-LAMP1 antibody (BA2787-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAMP1 antigen affinity purified polyclonal antibody (BA2787-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LAMP1 at approximately 90-120 kDa (Glycosylation). The expected band size for LAMP1 is at 42 kDa.
