Anti-FAM65B/RIPOR2 Antibody

筛选器： All WB FCM ELISA

• 

  Western blot analysis of anti-FAM65B/RIPOR2 antibody (A32101-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Daudi whole cell lysates,
  Lane 2: human HEL whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAM65B/RIPOR2 antigen affinity purified polyclonal antibody (A32101-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FAM65B/RIPOR2 at approximately 120 kDa. The expected band size for FAM65B/RIPOR2 is at 119 kDa.

• 

  Flow Cytometry analysis of HEL cells using anti-FAM65B/RIPOR2 antibody (A32101-1).
  Overlay histogram showing HEL cells stained with A32101-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAM65B/RIPOR2 Antibody (A32101-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti-FAM65B/RIPOR2 antibody (A32101-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Daudi whole cell lysates,
  Lane 2: human HEL whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAM65B/RIPOR2 antigen affinity purified polyclonal antibody (A32101-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FAM65B/RIPOR2 at approximately 120 kDa. The expected band size for FAM65B/RIPOR2 is at 119 kDa.

• 

  Flow Cytometry analysis of HEL cells using anti-FAM65B/RIPOR2 antibody (A32101-1).
  Overlay histogram showing HEL cells stained with A32101-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAM65B/RIPOR2 Antibody (A32101-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

•