Anti-ATP1B1 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC IF ICC/IF ELISA

A03469-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATP1B1 antigen affinity purified polyclonal antibody (A03469-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATP1B1 at approximately 50 kDa. The expected band size for ATP1B1 is at 35 kDa.
all(15)
IHC analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(15)
IHC analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(15)
IHC analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in a paraffin-embedded section of human Gall bladder adenosquamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(15)
IHC analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(15)
IHC analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(15)
IHC analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(15)
IHC analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(15)
IHC analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(15)
IHC analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(15)
IHC analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(15)
IF analysis using anti- ATP1B1 Antibody (A03469-1). detected in paraffin-embedded section of rat kidney tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(15)
IF analysis using anti- ATP1B1 Antibody (A03469-1). detected in paraffin-embedded section of mouse kidney tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(15)
ICC/IF analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1).
ATP1B1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-ATP1B1 Antibody (A03469-1) at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(15)
all(15)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ATP1B1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

ATP1B; ATP1B1; Beta 1 subunit of Na(+) K(+) ATPase; Na+/K+ ATPase beta 1 subunit

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, ICC/IF, ELISA

基因名称

ATP1B1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human ATP1B1 recombinant protein (Position: Q55-E271).

计算分子量

35 kDa

实际分子量

50 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Sodium/potassium-transporting ATPase subunit beta-1 is an enzyme that in humans is encoded by the ATP1B1 gene. The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.

Uniprot ID

P05026

文献引用格式

ATP1B1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03469-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ATPase Na+/K+ transporting subunit beta 1

蛋白名全称

Sodium/potassium-transporting ATPase subunit beta-1

实验方案>

实验步骤

引用文献(0)>

清除