Anti-MCM6 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM ELISA

A02755-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-MCM6 antibody (A02755-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human MOLT-4 whole cell lysates,
Lane 7: human HL-60 whole cell lysates,
Lane 8: rat testis tissue lysates,
Lane 9: rat ovary tissue lysates,
Lane 10: rat thymus tissue lysates,
Lane 11: mouse testis tissue lysates,
Lane 12: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MCM6 antigen affinity purified polyclonal antibody (A02755-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MCM6 at approximately 105 kDa. The expected band size for MCM6 is at 93 kDa.
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IHC analysis of MCM6 using anti-MCM6 antibody (A02755-1).
MCM6 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of MCM6 using anti-MCM6 antibody (A02755-1).
MCM6 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of MCM6 using anti-MCM6 antibody (A02755-1).
MCM6 was detected in a paraffin-embedded section of mouse lymphaden tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of MCM6 using anti-MCM6 antibody (A02755-1).
MCM6 was detected in a paraffin-embedded section of rat lymphaden tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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ICC/IF analysis of MCM6 using anti-MCM6 antibody (A02755-1).
MCM6 was detected in an immunocytochemical section of Caco-2 cells. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of A549 cells using anti-MCM6 antibody (A02755-1).
Overlay histogram showing A549 cells stained with A02755-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM6 Antibody (A02755-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-MCM6 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

MCG40308; MCM6; Mis5; P105MCM

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM, ELISA

基因名称

MCM6

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human MCM6 recombinant protein (Position: Q14-D821).

计算分子量

93 kDa

实际分子量

105 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

MCM6(Minichromosome maintenance, s. pombe, homolog of, 6) is a protein that in humans is encoded by the MCM6 gene. MCM6 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM genes were originally identified in yeast defective in minichromosome maintenance and have since been shown to play roles in the progression of the cell cycle; many are cell division control genes. The MCM6 gene is mapped on 2q21.3. Mcm 6 has recently been shown to interact strongly Cdt1 at defined residues, by mutating these target residues Wei et al. observed lack of Cdt1 recruitment of Mcm2-7 to the pre-RC. An approximately 200-kb region surrounding the C/T(-13910) polymorphism in MCM6 intron 13 functioned as an enhancer of the lactase gene promoter in intestinal cell culture.

Uniprot ID

Q14566

文献引用格式

MCM6 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02755-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

minichromosome maintenance complex component 6

蛋白名全称

DNA replication licensing factor MCM6

实验方案>

实验步骤

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