| Western blot (WB): | 1:500-2000 |
| ImmunoPrecipitation (IP): | 1:250-300 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |

Western blot analysis of 53BP2/TP53BP2 using anti-53BP2/TP53BP2 antibody (A02609-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-53BP2/TP53BP2 antigen affinity purified polyclonal antibody (A02609-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 53BP2/TP53BP2 at approximately 160 kDa. The expected band size for 53BP2/TP53BP2 is at 126 kDa.

IP analysis of 53BP2/TP53BP2 using anti-53BP2/TP53BP2 antibody (A02609-3) in K562 whole cell lysate.
Western blot analysis of 53BP2/TP53BP2 using anti- 53BP2/TP53BP2 antibody (A02609-3).
Lane 1: K562 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- 53BP2/TP53BP2 antibody in K562 whole cell lysate,
Lane 3: anti- 53BP2/TP53BP2 antibody (2μg) + K562 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- 53BP2/TP53BP2 antigen affinity purified polyclonal antibody (A02609-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 53BP2/TP53BP2 at approximately 160 kDa. The expected band size for 53BP2/TP53BP2 is at 126 kDa.

Western blot analysis of 53BP2/TP53BP2 using anti-53BP2/TP53BP2 antibody (A02609-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-53BP2/TP53BP2 antigen affinity purified polyclonal antibody (A02609-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 53BP2/TP53BP2 at approximately 160 kDa. The expected band size for 53BP2/TP53BP2 is at 126 kDa.

IP analysis of 53BP2/TP53BP2 using anti-53BP2/TP53BP2 antibody (A02609-3) in K562 whole cell lysate.
Western blot analysis of 53BP2/TP53BP2 using anti- 53BP2/TP53BP2 antibody (A02609-3).
Lane 1: K562 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- 53BP2/TP53BP2 antibody in K562 whole cell lysate,
Lane 3: anti- 53BP2/TP53BP2 antibody (2μg) + K562 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- 53BP2/TP53BP2 antigen affinity purified polyclonal antibody (A02609-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 53BP2/TP53BP2 at approximately 160 kDa. The expected band size for 53BP2/TP53BP2 is at 126 kDa.



