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Anti-Caveolin-3/CAV3 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC IF ELISA

A00990-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody (A00990-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human placenta tissue lysates,
    Lane 2: human U-87MG whole cell lysates,
    Lane 3: human Hela whole cell lysates,
    Lane 4: rat stomach tissue lysates,
    Lane 5: rat testis tissue lysates,
    Lane 6: mouse stomach tissue lysates,
    Lane 7: mouse testis tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caveolin-3/CAV3 antigen affinity purified polyclonal antibody (A00990-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caveolin-3/CAV3 at approximately 17 kDa. The expected band size for Caveolin-3/CAV3 is at 17 kDa.

    all(6)
  • IHC analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody (A00990-2).
    Caveolin-3/CAV3 was detected in a paraffin-embedded section of human skeletal muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • IF analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody (A00990-2).
    Caveolin-3/CAV3 was detected in a paraffin-embedded section of human cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(6)
  • IF analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody (A00990-2).
    Caveolin-3/CAV3 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(6)
  • IF analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody (A00990-2).
    Caveolin-3/CAV3 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(6)

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产品简介>

产品名称
Anti-Caveolin-3/CAV3 Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
CAV3; caveolin 3; Caveolin-3; LGMD1C; LQT9; M caveolin; VIP 21; VIP21
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, IF, ELISA
基因名称
CAV3
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human Caveolin-3/CAV3 recombinant protein (Position: M1-D55).
计算分子量
17 kDa
实际分子量
17 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites.
Uniprot ID
P56539  
文献引用格式
Caveolin-3/CAV3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00990-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunofluorescence (IF):1:50-400
ELISA:1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Caveolin-3/CAV3 Antibody

    筛选器: All WB IHC IF ELISA

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