Western blot analysis of ROCK1 using anti-ROCK1 antibody (A00722-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ROCK1 antigA03957-Aen affinity purified polyclonal antibody (A00722-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ROCK1 at approximately 158 kDa. The expected band size for ROCK1 is at 158 kDa.
IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5).
ROCK1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5).
ROCK1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5).
ROCK1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of ROCK1 using anti-ROCK1 antibody (A00722-5) and anti-Alpha Tubulin antibody (M03989-3).
ROCK1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of ROCK1 using anti-ROCK1 antibody (A00722-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ROCK1 antigA03957-Aen affinity purified polyclonal antibody (A00722-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ROCK1 at approximately 158 kDa. The expected band size for ROCK1 is at 158 kDa.
IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5).
ROCK1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5).
ROCK1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5).
ROCK1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of ROCK1 using anti-ROCK1 antibody (A00722-5) and anti-Alpha Tubulin antibody (M03989-3).
ROCK1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
Western blot analysis of ROCK1 using anti-ROCK1 antibody (A00722-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ROCK1 antigA03957-Aen affinity purified polyclonal antibody (A00722-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ROCK1 at approximately 158 kDa. The expected band size for ROCK1 is at 158 kDa.
IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5).
ROCK1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5).
ROCK1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5).
ROCK1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of ROCK1 using anti-ROCK1 antibody (A00722-5) and anti-Alpha Tubulin antibody (M03989-3).
ROCK1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-ROCK1 Antibody (A00722-5) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
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