Anti-p16INK4a/CDKN2A Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

A00016-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of p16INK4a/CDKN2A using anti-p16INK4a/CDKN2A antibody (A00016-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p16INK4a/CDKN2A antigen affinity purified polyclonal antibody (A00016-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for p16INK4a/CDKN2A at approximately 17 kDa. The expected band size for p16INK4a/CDKN2A is at 17 kDa.
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产品名称

Anti-p16INK4a/CDKN2A Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

ARF; ARF;CDKN2A; CDK4 inhibitor p16 INK4; CDK4 inhibitor p16INK4; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4a; MLM; MTS 1; MTS1; Multiple tumor suppressor 1; P16; p16 INK4; p16 INK4a; p16INK4; p16INK4a; P16-INK4A; TP16

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

CDKN2A

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human P16-INK4A/CDKN2A recombinant protein (Position: P48-D156).

计算分子量

17 kDa

实际分子量

17 kDa

成分

500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase.

Uniprot ID

P42771

文献引用格式

p16INK4a/CDKN2A Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00016-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin-dependent kinase inhibitor 2A

蛋白名全称

Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3

实验方案>

实验步骤

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